Ferroportin mRNA is down-regulated in granulosa and cervical cells from infertile women

Autor: Víctor Blasco, Marina Fontan, Antonio Romero-Ruiz, Francisco M. Pinto, Eva López-Navarro, Luz Candenas, José Manuel Fernández-Real, Wifredo Ricart, Manuel Tena-Sempere, Mònica Sabater-Masdeu, Manuel Fernández-Sánchez, José María Moreno-Navarrete, Francisco J. Ortega
Rok vydání: 2017
Předmět:
0301 basic medicine
Ferroportin
Tertiary Care Centers
0302 clinical medicine
In vitro fertilization
Gene expression
Cervical Atlas
Cation Transport Proteins
Ovarian granulosa cells
030219 obstetrics & reproductive medicine
biology
Obstetrics and Gynecology
Iron deficiency
Female
Oxidoreductases
Adult
Infertility
endocrine system
medicine.medical_specialty
Uterine cervical cells
Down-Regulation
Transferrin receptor
Fertilization in Vitro
in vitro fertilization
Young Adult
03 medical and health sciences
Hepcidins
Antigens
CD
Hepcidin
Internal medicine
Receptors
Transferrin
Sterility
medicine
Humans
RNA
Messenger
Ferritin
Granulosa Cells
urogenital system
medicine.disease
Ferritin light chain
Fertility
030104 developmental biology
Endocrinology
Reproductive Medicine
Spain
Case-Control Studies
Apoferritins
Ferritins
biology.protein
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2016.10.008
Popis: [Objective] To explore the relationship between iron and infertility by investigating iron-related gene expression in granulosa and uterine cervical cells.
[Design] Case-control study.
[Setting] Two tertiary hospitals.
[Patient(s)] Two independent cohorts of fertile (n = 18 and n = 17) and infertile (n = 31 and n = 35) women.
[Intervention(s)] In vitro fertilization.
[Main Outcome Measure(s)] Gene expression levels of ferritin light chain (FTL), ferritin heavy chain (FTH), transferrin receptor (TFRC), and ferroportin (SLC40A1) mRNA were analyzed in granulosa and cervical cells.
[Result(s)] In the first cohort, fertile and infertile women were similar in body mass index. Ferroportin mRNA levels were decreased in granulosa cells from infertile women in parallel with increased serum hepcidin levels. A positive association between ferroportin and TFRC mRNA, a gene associated with intracellular iron deficiency, was observed only in granulosa cells from fertile women. The major findings were replicated in a second independent cohort.
[Conclusion(s)] Ferroportin mRNAs and circulating hepcidin identify a subset of infertile women and may constitute a target for therapy.
Databáze: OpenAIRE
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