Molecular cytogenetic analysis of five newly established cervical cancer cell lines using G banding and fluorescence in situ hybridization
Autor: | Angela Thein, Eadie Heyderman, X. Han, Margaret Fox, Jennifer M. Parrington, Stuart J Steele |
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Rok vydání: | 1996 |
Předmět: |
Adult
Cancer Research medicine.medical_specialty Adenosquamous carcinoma G banding Uterine Cervical Neoplasms Chromosome Disorders Chromosome 9 Biology Mice Tumor Cells Cultured Genetics medicine Animals Humans Molecular Biology In Situ Hybridization Fluorescence Chromosome Aberrations medicine.diagnostic_test Cytogenetics Karyotype 3T3 Cells Middle Aged medicine.disease Molecular biology Chromosome Banding Squamous carcinoma Chromosomes Human Pair 1 Karyotyping Carcinoma Squamous Cell Adenocarcinoma Female Chromosomes Human Pair 3 Fluorescence in situ hybridization |
Zdroj: | Cancer Genetics and Cytogenetics. 91:28-36 |
ISSN: | 0165-4608 |
Popis: | Cervical tumors nearly all have complex karyotypes and more precise cytogenetic information is required to establish whether specific rearrangements occur, and if they are related to the type of HPV infection found. The karyotypes of five recently established cervical cancer cell lines, three from squamous cell carcinomas (two HPV 16 +ve and one HPV 18 +ve), one from an adenocarcinoma (HPV -ve), and one from an adenosquamous carcinoma (HPV 16 +ve), have been analysed using fluorescence in situ hybridization (FISH), with 23 chromosome specific paints, YACs and cosmids as probes, in addition to conventional G banding, in order to identify markers and clarify the breakpoints. Chromosomes 1 and 3 were rearranged in all cell lines. Breakpoints in the squamous lines were all in 3q. but in different regions. Small metacentrics involving chromosome 5 were a del(5q) in one line, and a t(X;5) in another, rather than i(5p). The region 6q21 was involved in three cases and chromosome 9 was rearranged in four. An i(8q) was found in three squamous carcinoma cell lines. Structural changes of 11q were found only in two cases, but a marker 11 representing amplification in the 11q14-22 region was duplicated in the adenosquamous line. |
Databáze: | OpenAIRE |
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