Free-radical Destruction of Sphingolipids Resulting in 2-hexadecenal Formation
| Autor: | Oleg I. Shadyro, I.P. Edimecheva, Alexandra Lisovskaya, Nadezda Amaegberi, G. N. Semenkova |
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| Rok vydání: | 2015 |
| Předmět: |
reactive oxygen species
chemistry.chemical_classification Reactive oxygen species sphingolipids Hypochlorous acid Radical HEK 293 cells Biological activity reactive chlorine species Biology Bioinformatics Biochemistry Sphingolipid 2-hexadecenal carbohydrates (lipids) myeloperoxidase chemistry.chemical_compound free-radical destruction chemistry Cell culture Biophysics Fragmentation (cell biology) Original Research |
| Zdroj: | Lipid insights |
| ISSN: | 1178-6353 |
| Popis: | The action of hypochlorous acid (HOCl) and γ-radiation on aqueous lysosphingolipid dispersions was found to produce 2-hexadecenal (Hex). This process includes the stages of formation of nitrogen-centered radicals from the starting molecules and the subsequent fragmentation of these radicals via the rupture of C–C and O–H bonds. These findings prove the existence of a nonenzymatic pathway of sphingolipid destruction leading to the formation of Hex, which possesses a wide spectrum of biological activity. Analysis of the effect of HOCl on transplantable rat glioma C6 cells and human embryonic kidney 293 cells points to the formation of Hex. This suggests that the described mechanism of free-radical destruction of sphingolipids may be replicated on cell culture under the stress of active chlorine forms. |
| Databáze: | OpenAIRE |
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