Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions
| Autor: | Izumi Kanisawa, Miyako Suzuki, Yoshihiro Sakuma, Yasuhiro Oikawa, Tomonori Nagamine, Hiroto Kamoda, Takahiro Ogura, Kenji Takahashi, Sumihisa Orita, Kazuyo Yamauchi, Kazuhisa Takahashi, Hideaki Fukuda, Masayuki Miyagi, Akihiro Tsuchiya, Tetsuhiro Ishikawa, Hiroki Sakai, Seiji Ohtori |
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| Jazyk: | angličtina |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Pathology Inflammatory mediator Inflammation Osteoarthritis 03 medical and health sciences 0302 clinical medicine Synovium medicine Orthopedics and Sports Medicine Synovial tissue Meniscal injury 030203 arthritis & rheumatology 030222 orthopedics business.industry Research medicine.disease Surgery Nerve growth factor Orthopedic surgery Tumor necrosis factor alpha medicine.symptom business Meniscal lesions |
| Zdroj: | Journal of Experimental Orthopaedics |
| ISSN: | 2197-1153 |
| DOI: | 10.1186/s40634-016-0041-9 |
| Popis: | Background Meniscal injuries are a risk factor for osteoarthritis (OA). While a mechanical pathway between meniscal injury and OA has been described, the biological effects of inflammation on this pathway have yet to be clarified. The aim of our study was to compare levels of specific inflammatory mediators, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and nerve growth factor (NGF), in injured and uninjured meniscal tissue and related knee joint synovium. Methods Tissue samples were obtained from 19 patients, 31.1 ± 13.6 years old, who underwent arthroscopic partial meniscectomy. For analysis, tissue samples were categorized into the following groups: injured meniscal site (IM), non-injured meniscal site (NIM), synovium ‘nearest’ the lesion (NS), and synovium from the opposite knee compartment, ‘farthest’ synovium (FS). Levels of inflammatory mediators were determined using enzyme-linked immunosorbent assay and between-group differences (IM and NIM; NS and FS) were evaluated using the Wilcoxon signed-rank test. The association between pre-operative pain score and the level of each inflammatory mediator was evaluated using Spearman’s correlation. Results Higher levels of TNF-α and IL-6 were identified in the IM tissue, compared to NIM (p |
| Databáze: | OpenAIRE |
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