Complete whole-genome sequence of the novel Pseudomonas species strain TUM18999, isolated from a patient with a burn wound in Japan
| Autor: | Kageto Yamada, Hinako Murakami, Kotaro Aoki, Masakazu Sasaki, Toshisuke Morita, Shinji Ujiie, Tatsuya Nagasawa, Yoshikazu Ishii, Toshiaki Ishii, Kazuhiro Tateda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Immunology Ceftazidime Genome Microbiology 03 medical and health sciences 0302 clinical medicine Japan Pseudomonas RNA Ribosomal 16S medicine Immunology and Allergy Humans Pseudomonas species 030212 general & internal medicine Phylogeny Whole genome sequencing Whole-genome sequencing biology Phylogenetic tree Broth microdilution Metallo-β-lactamase Average nucleotide identity biology.organism_classification 16S ribosomal RNA QR1-502 Alcaligenes Burns Genome Bacterial medicine.drug |
| Zdroj: | Journal of Global Antimicrobial Resistance, Vol 24, Iss, Pp 395-397 (2021) |
| ISSN: | 2213-7165 |
| Popis: | Objectives: Pseudomonas is a Gram-negative bacterial genus with numerous member species. In this study, using whole-genome sequencing, we characterized a novel Pseudomonas sp. strain TUM18999, isolated as a pathogen from a human patient. Methods: The TUM18999 strain was isolated from a patient’s burn wound. Minimum inhibitory concentrations (MICs) were determined using the broth microdilution method. The whole-genome sequence was obtained using Miseq and MinION, and we conducted phylogenetic analysis based on single nucleotide polymorphisms of the core genome. Results: Antimicrobial susceptibility testing revealed a high ceftazidime MIC (32 mg/L). Moreover, carbapenemase production was confirmed using the modified carbapenem inactivation method. We found that the complete genome of TUM18999 was 6,826,062 bp long, with 6175 coding sequences (CDS) and a DNA G+C content (non-plasmid) of 66.4 mol%. Consistent with the high similarities with the 16S rRNA sequences of P. otitidis MCC10330 (98.6%) and P. alcaligenes NBRC 14159 (99.2%), similarities ( |
| Databáze: | OpenAIRE |
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