Molecular prevalence of Chlamydia and Chlamydia-like bacteria in Tunisian domestic ruminant farms and their influencing risk factors
| Autor: | Imen Fendri, H. Jribi, Yaakoub Gharbi, Zouhir Mallek, A. Ben Slima, Mohamed Barkallah, Michel Gautier, Radhouane Gdoura |
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| Přispěvatelé: | Faculty of Sciences of Sfax, Toxicology- Microbiology and Environmental Health Laboratory (LR17ES06), Université de Sfax - University of Sfax, Toxicology- Microbiology and Environmental Health Laboratory (LR17ES06), 2Veterinary Research Center of Sfax, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Veterinary medicine sequence analysis polymerase chain reaction avortement induced abortion chlamydia Cattle Diseases law.invention 0403 veterinary science law Ruminant Risk Factors TaqMan PCR Prevalence mold contamination Chlamydia santé animale Microbiology and Parasitology 04 agricultural and veterinary sciences General Medicine sequencing Abortion Veterinary Microbiologie et Parasitologie [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology biodiversité génétique Animals Domestic contamination biologique Female analyse de séquences chlamydiae coculture DNA Bacterial Farms 040301 veterinary sciences 030106 microbiology Chlamydiae Sheep Diseases Biology Real-Time Polymerase Chain Reaction Microbiology 03 medical and health sciences Parachlamydia acanthamoebae Quarantine medicine Animals tunisie Chlamydiales Sheep General Veterinary General Immunology and Microbiology animal health abortion veterinary samples Chlamydia Infections biology.organism_classification medicine.disease tunisia élevage méthode pcr Herd Cattle |
| Zdroj: | Transboundary and emerging diseases Transboundary and emerging diseases, Wiley-Blackwell, 2018, 65 (2), pp.e329-e338. ⟨10.1111/tbed.12757⟩ Transboundary and Emerging Diseases 2 (65), e329-e338. (2018) |
| ISSN: | 1865-1674 1865-1682 |
| DOI: | 10.1111/tbed.12757⟩ |
| Popis: | Chlamydia and Chlamydia-like bacteria are well known to infect several organisms and may cause a wide range of diseases, particularly in ruminants. To gain insight into the prevalence and diversity of these intracellular bacteria, we applied a pan-Chlamydiales real-time PCR to 1,134 veterinary samples taken from 130 Tunisian ruminant herds. The true adjusted animal population-level prevalence was 12.9% in cattle, against 8.7% in sheep. In addition, the true adjusted herd-level prevalence of Chlamydiae was 80% in cattle and 25.5% in sheep. Chlamydiales from three family-level lineages were detected indicating a high biodiversity of Chlamydiales in ruminant herds. Our results showed that Parachlamydia acanthamoebae could be responsible for bovine and ovine chlamydiosis in central-eastern Tunisia. Multivariable logistic regression analysis at the animal population level indicated that strata and digestive disorders variables were the important risk factors of bovine and ovine chlamydiosis. However, origin and age variables were found to be associated with bovine and ovine chlamydiosis, respectively. At the herd level, risk factors for Chlamydia positivity were as follows: abortion and herd size for cattle against breeding system, cleaning frequency, quarantine, use of disinfectant and floor type for sheep. Paying attention to these risk factors will help improvement of control programs against this harmful zoonotic disease. |
| Databáze: | OpenAIRE |
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