Mitochondrial Calcium Uniporter (MCU) deficiency reveals an alternate path for Ca2+ uptake in photoreceptor mitochondria
| Autor: | James B. Hurley, Celia M. Bisbach, Frans Vinberg, Fatima Abbas, Deepak Poria, Rachel Hutto, Whitney M. Cleghorn, Susan E. Brockerhoff, Vladimir J. Kefalov |
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| Rok vydání: | 2020 |
| Předmět: |
Male
genetic structures Science Mitochondrion Neurotransmission Biochemistry Mitochondrial Membrane Transport Proteins Article Retina Sodium-Calcium Exchanger Mice Retinal Rod Photoreceptor Cells Animals Photoreceptor Cells Uniporter Zebrafish Mice Knockout Calcium metabolism Multidisciplinary biology Chemistry Biological Transport Energy metabolism Metabolism Zebrafish Proteins biology.organism_classification Mitochondria Cell biology Mice Inbred C57BL Calcium channels Retinal Cone Photoreceptor Cells Medicine Calcium Female sense organs Visual system Intracellular Visual phototransduction |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-19 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-72708-x |
| Popis: | Rods and cones use intracellular Ca2+ to regulate many functions, including phototransduction and neurotransmission. The Mitochondrial Calcium Uniporter (MCU) complex is thought to be the primary pathway for Ca2+ entry into mitochondria in eukaryotes. We investigate the hypothesis that mitochondrial Ca2+ uptake via MCU influences phototransduction and energy metabolism in photoreceptors using a mcu-/- zebrafish and a rod photoreceptor-specific Mcu-/- mouse. Using genetically encoded Ca2+ sensors to directly examine Ca2+ uptake in zebrafish cone mitochondria, we found that loss of MCU reduces but does not eliminate mitochondrial Ca2+ uptake. Loss of MCU does not lead to photoreceptor degeneration, mildly affects mitochondrial metabolism, and does not alter physiological responses to light, even in the absence of the Na+/Ca2+, K+ exchanger. Our results reveal that MCU is dispensable for vertebrate photoreceptor function, consistent with its low expression and the presence of an alternative pathway for Ca2+ uptake into photoreceptor mitochondria. |
| Databáze: | OpenAIRE |
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