A versatile and robust analytical method for hyaluronan quantification in crosslinked products and complex matrices
| Autor: | Riccardo Beninatto, Devis Galesso, Matteo Soato, Cristian Guarise, Fabio Bettella, Mauro Pavan |
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| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Fluorenylmethyloxycarbonyl chloride 010405 organic chemistry Depolymerization Organic Chemistry General Medicine engineering.material 010402 general chemistry Glucuronic acid 01 natural sciences Biochemistry High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Glucosamine Hyaluronic acid Carbohydrate Conformation engineering Acid hydrolysis Biopolymer Hyaluronic Acid |
| Zdroj: | Carbohydrate Research. 503:108314 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/j.carres.2021.108314 |
| Popis: | Hyaluronic acid (HA), a naturally occurring biopolymer composed of repeating units of d -glucuronic acid and N-acetyl-glucosamine, is widely used as principal component of drugs, medical devices, nutraceuticals and cosmetics. Chemical modifications of HA or the presence of unmodified HA in complex matrices often brings common analytical techniques to fail its identification or quantification. In this work, a specific method for the quantification of HA and HA derivatives was developed and tested. After strong acid hydrolysis, polysaccharide depolymerization and N-acetylglucosamine deacetylation, quantitatively yielded glucosamine residues were derivatized using Fluorenylmethyloxycarbonyl chloride (FMOC), separated and quantitated by means of HPLC equipped with UV detection. The method was partially validated according to ICH Q2(R1) and successfully applied on different viscosupplements composed by modified HA or medical devices containing unmodified HA in complex matrices. |
| Databáze: | OpenAIRE |
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