Reference values for peripheral blood B-lymphocyte subpopulations: a basis for multiparametric immunophenotyping of abnormal lymphocytes
| Autor: | J.L Marques, M. De Bruyere, H Benoit, Véronique Deneys, A. M. Mazzon |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Lymphoma B-Cell Immunology Population B-Lymphocyte Subsets Lymphoproliferative disorders chemical and pharmacologic phenomena Immunoglobulin D Fluorescence Immunophenotyping Diagnosis Differential Reference Values immune system diseases hemic and lymphatic diseases Humans Immunology and Allergy Medicine Lymphocyte Count education B cell education.field_of_study biology business.industry CD23 hemic and immune systems Middle Aged Flow Cytometry medicine.disease Leukemia Lymphocytic Chronic B-Cell Antigens Differentiation B-Lymphocyte medicine.anatomical_structure biology.protein Female CD5 business Abnormal Lymphocyte |
| Zdroj: | Journal of Immunological Methods. 253:23-36 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/s0022-1759(01)00338-6 |
| Popis: | BACKGROUND AND OBJECTIVES: Immunophenotyping has become a useful tool for the differential diagnosis of chronic B-cell lymphoproliferative disorders. The aim of this work was to determine reference values of normal B-cell subpopulations. MATERIAL AND METHODS: Blood samples from 38 healthy volunteers were analyzed by multidimensional flow cytometry, using a panel of directly conjugated antibodies. Results were expressed as percent of positive B cells and as median fluorescence intensity, an indirect assessment of the expression level. RESULTS: CD20, CD22, CD24, CD40, CD79a, CD79b, FMC7, CD11a, CD18, CD44 were positive in the whole B cell population, whereas CD10, CD86, CD103, CD154 and FasL were almost absent from the B-lymphocyte population. 75% were IgD positive. The kappa/lambda ratio was 1.5. CD5, CD23, CD25, CD38, CD43, CD54, CD62L, CD80 and CD95 were positive in different B-cell subpopulations. The utility of all these markers in the differential diagnosis of chronic B-cell lymphoproliferative disorders is discussed. CONCLUSION: In order to interpret a pathological immunophenotype, it is necessary to refer to quantitative and qualitative values of normal B-cell subpopulations. |
| Databáze: | OpenAIRE |
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