Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross
Autor: | Zhenglin Du, Jun Xiao, Lu Wang, Chao Mai, Jing Luo, Wei Duan, Tao Li, Yun Gao, Jing Wang, Zhou Wei, Qinbo Qin, Yi Zhou, Chun Zhang, Min Tao, Feng Huang, Robert W. Murphy, Ya-Ping Zhang, Rurong Zhao, Axel Meyer, Kaikun Luo, Shuo Yuan, Hao Feng, Xiaochuan Liu, Zhao-Li Ding, Yan-Qin Wang, Yun Liu, Zhen-Yan Song, Yubao Chen, Shaojun Liu, Jing Chai, Li Ren, Jun Wang, Li Zhong, Guoliang Lin, Xuemei Lu, Huan Zhong, Bin Lu |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Genomics Chimeric gene Biology Genome Chromosomes 03 medical and health sciences Meiosis Goldfish ddc:570 Homologous chromosome Animals Gene Crosses Genetic In Situ Hybridization Fluorescence Genetics Ploidies Multidisciplinary Gene Expression Profiling fungi food and beverages Biological Sciences genomic DNA 030104 developmental biology sense organs Ploidy |
Zdroj: | Proceedings of the National Academy of Sciences. 113:1327-1332 |
ISSN: | 1091-6490 0027-8424 |
Popis: | Polyploidy is much rarer in animals than in plants but it is not known why. The outcome of combining two genomes in vertebrates remains unpredictable, especially because polyploidization seldom shows positive effects and more often results in lethal consequences because viable gametes fail to form during meiosis. Fortunately, the goldfish (maternal) × common carp (paternal) hybrids have reproduced successfully up to generation 22, and this hybrid lineage permits an investigation into the genomics of hybridization and tetraploidization. The first two generations of these hybrids are diploids, and subsequent generations are tetraploids. Liver transcriptomes from four generations and their progenitors reveal chimeric genes (>9%) and mutations of orthologous genes. Characterizations of 18 randomly chosen genes from genomic DNA and cDNA confirm the chimera. Some of the chimeric and differentially expressed genes relate to mutagenesis, repair, and cancer-related pathways in 2nF1. Erroneous DNA excision between homologous parental genes may drive the high percentage of chimeric genes, or even more potential mechanisms may result in this phenomenon. Meanwhile, diploid offspring show paternal-biased expression, yet tetraploids show maternal-biased expression. These discoveries reveal that fast and unstable changes are mainly deleterious at the level of transcriptomes although some offspring still survive their genomic abnormalities. In addition, the synthetic effect of genome shock might have resulted in greatly reduced viability of 2nF2 hybrid offspring. The goldfish × common carp hybrids constitute an ideal system for unveiling the consequences of intergenomic interactions in hybrid vertebrate genomes and their fertility. published |
Databáze: | OpenAIRE |
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