Electrochemical Single‐Cell Protein Therapeutics Using a Double‐Barrel Nanopipette
| Autor: | Xiao‐Mei Shi, Yi‐Tong Xu, Bing‐Yu Zhou, Bing Wang, Si‐Yuan Yu, Wei‐Wei Zhao, Dechen Jiang, Hong‐Yuan Chen, Jing‐Juan Xu |
|---|---|
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Angewandte Chemie. 135 |
| ISSN: | 1521-3757 0044-8249 |
| DOI: | 10.1002/ange.202215801 |
| Popis: | Single-cell protein therapeutics is expected to promote our in-depth understanding of how does a specific protein with therapeutic dosing treat the cell without population averaging. However, it has not yet been tackled by current single-cell nanotools. We address this challenge by utilization of a double-barrel nanopipette, in which one lumen was used for electroosmotic cytosolic protein delivery and the other was customized for ionic evaluation of the consequence. Upon injection of protein DJ-1 via delivery lumen, upregulation of the antioxidant protein could protect neural PC-12 cells against oxidative stress from phorbol myristate acetate exposure, which could be transduced by targeting the cytosolic hydrogen peroxide by the detecting lumen. This work has devised a nanotool for single-cell protein therapeutics and provided a perspective for futuristic single-cell therapeutics involving different therapeutic modalities e.g. peptides, enzymes and nucleic acids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|