Genome-wide gene expression profiling of low-dose, long-term exposure of human osteosarcoma cells to bisphenol A and its analogs bisphenols AF and S
| Autor: | Janja Marc, S. Jurkovic Mlakar, L. Peterlin Mašič, Anja Fic, M. Sollner Dolenc, Vid Mlakar, Karin Broberg, P. Juvan |
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| Rok vydání: | 2015 |
| Předmět: |
endocrine system
medicine.medical_specialty Microarray Biology Toxicology Isoprenoid biosynthetic process Immune system Phenols Cell Line Tumor Internal medicine Gene expression medicine Humans Sulfones Benzhydryl Compounds Gene Oligonucleotide Array Sequence Analysis Osteosarcoma urogenital system Gene Expression Profiling General Medicine Molecular biology Gene expression profiling Endocrinology Endocrine disruptor hormones hormone substitutes and hormone antagonists TXNIP |
| Zdroj: | Toxicology in Vitro. 29:1060-1069 |
| ISSN: | 0887-2333 |
| Popis: | The bisphenols AF (BPAF) and S (BPS) are structural analogs of the endocrine disruptor bisphenol A (BPA), and are used in common products as a replacement for BPA. To elucidate genome-wide gene expression responses, estrogen-dependent osteosarcoma cells were cultured with 10 nM BPA, BPAF, or BPS, for 8 h and 3 months. Genome-wide gene expression was analyzed using the Illumina Expression BeadChip. Three months exposure had significant effects on gene expression, particularly for BPS, followed by BPAF and BPA, according to the number of differentially expressed genes (1980, 778, 60, respectively), the magnitude of changes in gene expression, and the number of enriched biological processes (800, 415, 33, respectively) and pathways (77, 52, 6, respectively). 'Embryonic skeletal system development' was the most enriched bone-related process, which was affected only by BPAF and BPS. Interestingly, all three bisphenols showed highest down-regulation of genes related to the cardiovascular system (e.g., NPPB, NPR3, TXNIP). BPA only and BPA/BPAF/BPS also affected genes related to the immune system and fetal development, respectively. For BPAF and BPS, the 'isoprenoid biosynthetic process' was enriched (up-regulated genes: HMGCS1, PDSS1, ACAT2, RCE1, DHDDS). Compared to BPA, BPAF and BPS had more effects on gene expression after long-term exposure. These findings stress the need for careful toxicological characterization of BPA analogs in the future. |
| Databáze: | OpenAIRE |
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