The role of TCPTP on leptin effects on astrocyte morphology
| Autor: | José Antunes-Rodrigues, Hellen Veida-Silva, Beatriz C. Borges, Maria Célia Jamur, Lucila Leico Kagohara Elias, Lucas Kniess Debarba, F. M. V. Vechiato |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Leptin Lipopolysaccharides medicine.medical_treatment Hypothalamus Connexin 030209 endocrinology & metabolism ROEDORES Protein tyrosine phosphatase Biochemistry Energy homeostasis 03 medical and health sciences 0302 clinical medicine Endocrinology medicine Connexin 30 Animals Rats Wistar Molecular Biology Cells Cultured Protein Tyrosine Phosphatase Non-Receptor Type 1 Protein Tyrosine Phosphatase Non-Receptor Type 2 Chemistry Gap junction Organ Size medicine.disease Cell biology Astrogliosis Rats Up-Regulation 030104 developmental biology medicine.anatomical_structure Cytokine Animals Newborn Astrocytes Connexin 43 Cytokines hormones hormone substitutes and hormone antagonists Astrocyte |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Leptin and LPS has been implicated in the development of hypothalamic astrogliosis in rodents. Astrocytes, which are interconnected by gap junction proteins, have emerged as important players in the control of energy homeostasis exerted by the hypothalamus. To investigate the hypothesis of action of T-cell protein tyrosine phosphatase (TCPTP) on the astrocyte morphology, astrocytes from the hypothalamus of one-day-old rats were stimulated with leptin and LPS (used as a positive control). Leptin and LPS induced a marked increase in astrocyte size, an increase in Ptpn2 (TCPTP gene) and gap junction alpha-1 protein, - Gja1 (connexin 43 - CX43 gene) mRNA expression and a decrease in gap junction protein, alpha 6 - Gja6 (CX30 gene) mRNA expression. Remarkably, these effects on astrocytes morphology and connexins were prevented by Ptpn2 siRNA. Astrocytes are known to produce cytokines; here we show that TCPTP acts as an important regulator of the cytokines and it possesses a reciprocal interplay with protein tyrosine phosphatase 1B (PTP1B). Our findings demonstrate that leptin and LPS alter astrocyte morphology by increasing TCPTP, which in turn modulates connexin 30 (CX30) and connexin 43 (CX43) expression. TCPTP and PTP1B seem to act in the regulation of cytokine production in astrocytes. |
| Databáze: | OpenAIRE |
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