Deamination of L-Thyroxine by Oxidase and Transaminase of Rat Kidney Mitochondria
| Autor: | Shiro Tsuchiya, T. S. Danowski, Minoru Nakano, Shojiro Shimizu, Saburo Yamazoe |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
chemistry.chemical_classification
Thyroid Hormones Ammonium sulfate Oxidase test Transamination Research Deamination Oxidative deamination Metabolism Kidney Mitochondria Rats Transaminase Thyroxine chemistry.chemical_compound Endocrinology Biochemistry chemistry Pyridoxal Phosphate Ketoglutaric Acids Pyridoxal phosphate Oxidoreductases Transaminases |
| Zdroj: | Endocrinology. 73:237-243 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Two soluble enzyme preparations, one which effects oxidative deamination of thyroxine and another which catalyzes transamination between thyroxine and a-ketoglutarate in the presence of pyridoxal phosphate, have been obtained from extracts of rat kidney mitochondria disintegrated by sonic treatment. After successive fractionation of the extracts with ammonium sulfate, the oxidase and the transaminase activities were largely concentrated in precipitates obtained with 33–50 and 50–70 % ammonium sulfate, respectively. These oxidase and transaminase activities appear to be maximal at a pH of approximately 8.0 and 6.5, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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