Factors Known to Influence the Development of Necrotizing Enterocolitis to Modify Expression and Activity of Intestinal Alkaline Phosphatase in a Newborn Neonatal Rat Model
Autor: | Rebecca M. Rentea, Ben E. Biesterveld, David M. Gourlay, Matthea J. Rentea, Jennifer L. Liedel |
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Rok vydání: | 2018 |
Předmět: |
medicine.medical_specialty
Population Infant Premature Diseases Breast milk Enteral administration Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Enterocolitis Necrotizing Risk Factors 030225 pediatrics Internal medicine Animals Humans Medicine education Enterocolitis education.field_of_study Cesarean Section business.industry Infant Newborn Alkaline Phosphatase medicine.disease Infant Formula Rats Isoenzymes body regions Endocrinology Animals Newborn Infant formula 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Necrotizing enterocolitis Alkaline phosphatase Surgery Enterocyte differentiation medicine.symptom business Biomarkers |
Zdroj: | European Journal of Pediatric Surgery. 29:290-297 |
ISSN: | 1439-359X 0939-7248 |
DOI: | 10.1055/s-0038-1646959 |
Popis: | Introduction Prematurity, formula feeding, and early weaning strongly influence enterocyte differentiation. Intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, is one enzyme that is affected by these factors. IAP supplementation decreases the severity of necrotizing enterocolitis (NEC) injury. We, therefore, hypothesized that prematurity predisposes this population to NEC due to IAP deficiency and investigated IAP expression and function in a neonatal rat model. Materials and Methods Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on consecutive days of life both after vaginal or cesarean birth and following either breast or formula feeding. Results Compared with controls, cesarean delivery and formula feeding are associated with lower levels of IAP. The formula-fed pups continued to have low baseline IAP activity. Neither prematurity nor formula feeding led to differences of intestinal injury. Conclusion Prematurity and formula feeding are associated with inhibition of IAP expression and activity. Both may increase the risk of NEC and early enteral supplementation of IAP to newborns at risk of NEC may be of therapeutic benefit. |
Databáze: | OpenAIRE |
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