Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
| Autor: | Carlos André Zucco, Flábio R. Araújo, Silvio Arruda Vasconcellos, G. M. S. Rosinha, Walfrido Moraes Tomas, Anahi Souto Vieira, Paulo Andre Lima-Borges, Carina Elisei de Oliveira, Cleber O. Soares, Ubiratan Piovezan, Guilherme Mourão, Aiesca Oliveira Pellegrin, Ana Cristina Reis Lacerda |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Serotype Male Veterinary medicine lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 animal diseases lcsh:QR1-502 Pampas deer Polymerase Chain Reaction lcsh:Microbiology law.invention Serology Environmental protection law Leptospira Direct agglutination test Agglutination Tests Dry season Animals Leptospirosis Polymerase chain reaction Phylogeny biology Deer Leptospira spp Pantanal biology.organism_classification Antibodies Bacterial Leptospira interrogans serovar pomona PCR MAT Wetlands pampas deer Female Seasons Leptospira interrogans Brazil |
| Zdroj: | Repositório Institucional da UnB Universidade de Brasília (UnB) instacron:UNB Memórias do Instituto Oswaldo Cruz., Vol 106, Iss 6, Pp 763-768 (2011) Memórias do Instituto Oswaldo Cruz, Volume: 106, Issue: 6, Pages: 763-768, Published: SEP 2011 ResearcherID Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed. |
| Databáze: | OpenAIRE |
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