Expression of active B-Raf proto-oncogene in kidney collecting ducts induces cyst formation in normal mice and accelerates cyst growth in mice with polycystic kidney disease
| Autor: | Stephen C. Parnell, Archana Raman, Yan Zhang, Emily A. Daniel, Yuqiao Dai, Aditi Khanna, Gail A. Reif, Jay L. Vivian, Timothy A. Fields, Darren P. Wallace |
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| Rok vydání: | 2022 |
| Předmět: |
Proto-Oncogene Proteins B-raf
Mitogen-Activated Protein Kinase Kinases Cysts Mice Transgenic Receptors Cell Surface Polycystic Kidney Autosomal Dominant Fibrosis Arginine Vasopressin Mice Nephrology Proliferating Cell Nuclear Antigen Proto-Oncogenes Cyclic AMP Animals Kidney Tubules Collecting Polycystic Kidney Autosomal Recessive |
| Zdroj: | Kidney International. 102:1103-1114 |
| ISSN: | 0085-2538 |
| Popis: | Polycystic kidney disease (PKD) is characterized by the formation and progressive enlargement of fluid-filled cysts due to abnormal cell proliferation. Cyclic AMP agonists, including arginine vasopressin, stimulate ERK-dependent proliferation of cystic cells, but not normal kidney cells. Previously, B-Raf proto-oncogene (BRAF), a MAPK kinase kinase that activates MEK-ERK signaling, was shown to be a central intermediate in the cAMP mitogenic response. However, the role of BRAF on cyst formation and enlargement in vivo had not been demonstrated. To determine if active BRAF induces kidney cyst formation, we generated transgenic mice that conditionally express BRAF |
| Databáze: | OpenAIRE |
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