Osteoblast mineralization requires β1 integrin/ICAP-1–dependent fibronectin deposition
| Autor: | Genevieve Chevalier, Inaam A. Nakchbandi, Molly Brunner, Angélique Millon-Frémillon, Daniel Bouvard, Marc R. Block, Deane F. Mosher, Corinne Albiges-Rizo |
|---|---|
| Přispěvatelé: | Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Dynamique des systèmes d'adhérence et différenciation (DySAD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Molecular Medicine [Martinsreid], Max Planck Institute of Biochemistry (MPIB), Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, School of Medicine and Public Health, University of Wisconsin-Madison, Pro-A INSERM, Ligue Nationale Contre le cancer (DB), NIH HL21644 (DM), Block, Marc, Max-Planck-Institut für Biochemie = Max Planck Institute of Biochemistry (MPIB) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
ICAP-1
integrin Cellular differentiation Integrin Immunology Muscle Proteins [SDV.BC]Life Sciences [q-bio]/Cellular Biology Mineralization (biology) Article osteogenesis Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine Calcification Physiologic medicine Immunology and Allergy Animals mineralization Cytoskeleton [SDV.BC] Life Sciences [q-bio]/Cellular Biology Research Articles 030304 developmental biology Cell Proliferation 0303 health sciences kindlin Osteoblasts biology Integrin beta1 β1 integrin Intracellular Signaling Peptides and Proteins Correction Osteoblast Cell Differentiation Cell Biology Fibronectins Cell biology Extracellular Matrix Fibronectin Cytoskeletal Proteins medicine.anatomical_structure biology.protein fibrilogenesis Type I collagen 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | The Journal of Cell Biology Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2011, 194 (2), pp.307-22. ⟨10.1083/jcb.201007108⟩ Journal of Cell Biology, 2011, 194 (2), pp.307-22. ⟨10.1083/jcb.201007108⟩ |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | ICAP-1 prevents recruitment of kindlin-2 to β1 integrin to control dynamics of fibrillar adhesion sites, fibronectin deposition, and osteoblast mineralization during bone formation. The morphogenetic and differentiation events required for bone formation are orchestrated by diffusible and insoluble factors that are localized within the extracellular matrix. In mice, the deletion of ICAP-1, a modulator of β1 integrin activation, leads to severe defects in osteoblast proliferation, differentiation, and mineralization and to a delay in bone formation. Deposition of fibronectin and maturation of fibrillar adhesions, adhesive structures that accompany fibronectin deposition, are impaired upon ICAP-1 loss, as are type I collagen deposition and mineralization. Expression of β1 integrin with a mutated binding site for ICAP-1 recapitulates the ICAP-1–null phenotype. Follow-up experiments demonstrated that ICAP-1 negatively regulates kindlin-2 recruitment onto the β1 integrin cytoplasmic domain, whereas an excess of kindlin-2 binding has a deleterious effect on fibrillar adhesion formation. These results suggest that ICAP-1 works in concert with kindlin-2 to control the dynamics of β1 integrin–containing fibrillar adhesions and, thereby, regulates fibronectin deposition and osteoblast mineralization. |
| Databáze: | OpenAIRE |
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