Quantitation of amyloid-β peptides in biological milieu using a novel homogeneous time-resolved fluorescence (HTRF) assay
| Autor: | Earl E. Clarke, Mark S. Shearman |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Time Factors Fluoroimmunoassay Antigen-Antibody Complex Alzheimer Disease Antibody Specificity medicine Amyloid precursor protein Humans Bovine serum albumin Cells Cultured Amyloid beta-Peptides medicine.diagnostic_test biology Chemistry General Neuroscience medicine.disease Amyloid β peptide Culture Media Protein Structure Tertiary Biochemistry Homogeneous Cell culture Immunoassay biology.protein Alzheimer's disease Time-resolved spectroscopy Peptides Blood Chemical Analysis |
| Zdroj: | Journal of Neuroscience Methods. 102:61-68 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/s0165-0270(00)00280-6 |
| Popis: | Many of the recent advances in the understanding of the pathological processes underlying Alzheimer's disease have come about as a result of the development of assays that can specifically quantitate in biological milieu amyloid-beta (A beta) peptides ending at amino-acid positions Ala-42 (A beta(42)) and Val-40 (A beta(40)). The existing technologies, however, although proven in their utility are limited in their application with regards to sample manipulation and suitability for high-throughput screening. To overcome these limitations, in this report we describe the development of a novel homogeneous time-resolved fluorescence (HTRF) immunoassay for A beta(42) and A beta(40) peptides. This assay has the sensitivity, selectivity and dynamic range to allow specific, direct quantitation of A beta peptides in cell culture medium, plasma, cerebrospinal fluid and brain tissue extracts, and has the major advantage of minimising sample manipulation and its inherent inaccuracies. |
| Databáze: | OpenAIRE |
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