Comparison of different strategies towards the chemical synthesis of long-chain scorpion toxin AaH-II
| Autor: | Yani Liu, Yun-Kun Qi, Qian Qu, Lei Liu, Yu-Lei Li, Ke-Wei Wang, Ge-Min Fang |
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| Rok vydání: | 2021 |
| Předmět: |
Androctonus australis
Scorpion Venoms Peptide Biochemistry Chemical synthesis Scorpions chemistry.chemical_compound Structural Biology Drug Discovery Peptide synthesis Molecule Animals Molecular Biology Pharmacology chemistry.chemical_classification Scorpion toxin biology Chemistry Organic Chemistry Sodium General Medicine Native chemical ligation biology.organism_classification Molecular Medicine Peptides |
| Zdroj: | Journal of peptide science : an official publication of the European Peptide SocietyREFERENCES. 28(2) |
| ISSN: | 1099-1387 |
| Popis: | Long-chain scorpion toxin AaH-II isolated from Androctonus australis Hector can selectively inhibit mammalian voltage-gated sodium ion channel Nav 1.7 responsible for pain sensation. Efficient chemical synthesis of AaH-II and its derivatives is beneficial to the study of the function and mechanism of Nav 1.7 and the development of potential peptide inhibitors. Herein, we compared three different strategies, namely, direct solid-phase peptide synthesis, hydrazide-based two-segment native chemical ligation, and hydrazide-based three-segment native chemical ligation for the synthesis of AaH-II. The hydrazide-based two-segment native chemical ligation affords the target toxin with the optimal efficiency, which provides a practically robust procedure for the preparation of tool molecules derived from AaH-II to study the biological functions and modulation of Nav 1.7. Our work highlights the importance of selecting suitable segment condensation approach in the chemical synthesis of protein toxins. |
| Databáze: | OpenAIRE |
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