Co-culture of hepatocytes and fibroblasts by micropatterned immobilization of beta-galactose derivatives
| Autor: | Yoshihiro Ito, Geel Ja Kim, Oh Hyeong Kwon, Inn-Kyu Kang |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Materials science Cell Survival Surface Properties Biophysics Bioengineering Biocompatible Materials Allylamine Biomaterials Extracellular matrix chemistry.chemical_compound Mice Materials Testing Cell Adhesion Polyamines Animals Polymethyl Methacrylate Methyl methacrylate biology Tissue Engineering Substrate (chemistry) Galactose 3T3 Cells Fibroblasts Lactobionic acid Coculture Techniques Rats Fibronectin Biochemistry chemistry Mechanics of Materials Cell culture Ceramics and Composites biology.protein Hepatocytes Adsorption Layer (electronics) |
| Zdroj: | Biomaterials. 25(18) |
| ISSN: | 0142-9612 |
| Popis: | Photosensitive poly(allylamine) containing β -galactose moieties in the side chain (LPAN 3 ) was prepared by the reaction of poly(allylamine) with lactobionic acid and azidobenzoic acid. To create micropatterned surfaces, a LPAN 3 -coated poly(methyl methacrylate) (PMMA) substrate was irradiated with an ultraviolet lamp under a photomask. The presence of a LPAN 3 layer on the substrate was confirmed using an electron spectroscopy for chemical analysis. Micropatterned cell culture was carried out by seeding hepatocytes and/or fibroblasts on the substrate. As a result, hepatocytes and fibroblasts adhered only to the LPAN 3 and PMMA lane, respectively. Co-culture on the stripe patterned substrate was carried out by the first cell seeding of hepatocytes and subsequently by the second cell seeding of fibroblasts. The co-cultured cells produced extracellular matrix such as fibronectin, suggesting a biological function. |
| Databáze: | OpenAIRE |
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