Co-Suppression of NbClpC1 and NbClpC2, Encoding Clp Protease Chaperons, Elicits Significant Changes in the Metabolic Profile of Nicotiana benthamiana
| Autor: | Kwang-Hyun Baek, Md. Sarafat Ali |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteases clp protease Nicotiana benthamiana virus-induced gene silencing Plant Science Pentose phosphate pathway 01 natural sciences 03 medical and health sciences lcsh:Botany co-suppression Nucleotide Glycolysis Plastid Peptide sequence Ecology Evolution Behavior and Systematics metabolites chemistry.chemical_classification Ecology biology fungi food and beverages biology.organism_classification lcsh:QK1-989 Citric acid cycle 030104 developmental biology chemistry Biochemistry clpc1 clpc2 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 2, p 259 (2020) Plants Volume 9 Issue 2 |
| ISSN: | 2223-7747 |
| Popis: | Metabolites in plants are the products of cellular metabolic processes, and their differential amount can be regarded as the final responses of plants to genetic, epigenetic, or environmental stresses. The Clp protease complex, composed of the chaperonic parts and degradation proteases, is the major degradation system for proteins in plastids. ClpC1 and ClpC2 are the two chaperonic proteins for the Clp protease complex and share more than 90% nucleotide and amino acid sequence similarities. In this study, we employed virus-induced gene silencing to simultaneously suppress the expression of ClpC1 and ClpC2 in Nicotiana benthamiana (NbClpC1/C2). The co-suppression of NbClpC1/C2 in N. benthamiana resulted in aberrant development, with severely chlorotic leaves and stunted growth. A comparison of the control and NbClpC1/C2 co-suppressed N. benthamiana metabolomes revealed a total of 152 metabolites identified by capillary electrophoresis time-of-flight mass spectrometry. The co-suppression of NbClpC1/C2 significantly altered the levels of metabolites in glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, and the purine biosynthetic pathway, as well as polyamine and antioxidant metabolites. Our results show that the simultaneous suppression of ClpC1 and ClpC2 leads to aberrant morphological changes in chloroplasts and that these changes are related to changes in the contents of major metabolites acting in cellular metabolism and biosynthetic pathways. |
| Databáze: | OpenAIRE |
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