Protonated polynucleotide structures

Autor: Axel G. Lezius, Marie-Thérèse Sarocchi, Christian Marck, Danielle Thiele, Wilhelm Guschlbauer
Rok vydání: 1973
Předmět:
Zdroj: Molecular Biology Reports. 1:155-160
ISSN: 1573-4978
0301-4851
DOI: 10.1007/bf00357156
Popis: The CD spectra of three polydeoxyribonucleotide complexes, poly(dG-C). poly(dG-C), poly(dA-G). poly(dT-C) and poly(dA-C). poly(dT-G) have been studied as a function of pH at 25°C. Only poly(dA-G). poly(dT-C) showed large hysteresis, analogous to that observed for poly(dG). poly(dC), while the alternating pur-p-pyr containing polymers reversible titration curves exhibited. The hysteretic form R of poly(dA-G). poly(dT-C) is metastable at neutrality and reverts spontaneously and irreversibly to the neutral from N upon heating to about 60°. A whole titration cycle to pH 2.5 and back to neutrality has to be performed in order to obtain the metastable form R again.
Databáze: OpenAIRE
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