Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles
| Autor: | Luminiţa Măruţescu, Nicoleta Constantin, Sorina Mitrea, Liliana Burlibaşa, Eduard Marius Lungulescu, Marcela Popa, Magdalena Lungu, Gabriela Sbarcea, Mariana Carmen Chifiriuc, Coralia Bleotu, Anca Hermenean |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
Scanning electron microscope General Chemical Engineering Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences Chemical synthesis lcsh:Chemistry chemistry.chemical_compound Intestinal mucosa mental disorders Fourier transform infrared spectroscopy health care economics and organizations Wurtzite crystal structure Chemistry technology industry and agriculture General Chemistry respiratory system 021001 nanoscience & nanotechnology Polyelectrolyte 0104 chemical sciences Silver nitrate lcsh:QD1-999 0210 nano-technology Nuclear chemistry |
| Zdroj: | Arabian Journal of Chemistry, Vol 13, Iss 2, Pp 4180-4197 (2020) |
| ISSN: | 1878-5352 |
| Popis: | Silver-zinc oxide nanoparticles (AgZnO NPs) were chemically synthesized by the deposition of Ag NPs on the surface of ZnO NPs using silver nitrate, three types of anionic polyelectrolytes and citric acid as reagents. The Wavelength Dispersive X-ray Fluorescence (WDXRF) spectrometry of AgZnO NPs revealed 0.41–0.69 wt% Ag, and balance ZnO. The existence of Ag NPs on the surface of ZnO NPs with hexagonal wurtzite structure was highlighted by X-ray Diffraction (XRD) analysis, scanning electron microscopy (SEM), and Ultraviolet–Visible (UV–Vis) spectroscopy. The diffuse reflectance absorption of AgZnO NPs in the visible light region increased with the increase of Ag NPs content. The Fourier Transform Infrared (FTIR) spectrometry revealed no chemical bonding between Ag NPs and ZnO NPs and confirmed the presence of functional groups characteristic to ZnO and carboxylic acid salts. The newly synthesized AgZnO NPs displayed antimicrobial activity against all the tested medically relevant pathogens, with minimal (biofilm) inhibitory concentrations ranging from 1.875 mg/mL to 7.5 mg/mL. Although the in vitro genotoxicity assay revealed a relatively high micronuclei index, the in vivo micronucleus (MN) test revealed a low MN frequency in animals treated with AgZnO NPs. The histopathological analysis revealed non-significant structural changes of the hepatic parenchyma, renal cortex and intestinal mucosa and minimal inflammatory reactions. The AgZnO NPs administration induced TUNEL positive nuclei of Kupffer cells in the liver parenchyma. The present study shows that the newly synthesized AgZnO NPs are active against planktonic and adherent microorganisms and could be exploited to develop novel antimicrobial strategies for the biotechnology and biomedical fields. Easy scalability of the developed chemical synthesis is a major advantage in producing large batches of AgZnO NPs with reproducible properties. Keywords: Chemically synthesized AgZnO nanoparticles, Anionic polyelectrolytes, Medically relevant pathogens, Antimicrobial activity, Genotoxicity, Immunohistopathology |
| Databáze: | OpenAIRE |
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