Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium
| Autor: | Marianne Goodchild, Muhammad Iqbal, Duncan J. Maskell, Richard K. Beal, Yvonne Boyd, Nat Bumstead, Paul Wigley, John J. Young, Victoria J. Philbin, Adrian Smith, G. S. K. Withanage, Paul A. Barrow, Ian McConnell |
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| Rok vydání: | 2005 |
| Předmět: |
Salmonella typhimurium
Salmonella Molecular Sequence Data Immunology Mutant Receptors Cell Surface medicine.disease_cause Microbiology medicine Animals Amino Acid Sequence RNA Messenger Receptor Host Response and Inflammation Salmonella Infections Animal Toll-like receptor Membrane Glycoproteins biology Interleukin-6 Toll-Like Receptors Pattern recognition receptor biology.organism_classification Toll-Like Receptor 5 Infectious Diseases Salmonella enterica TLR5 biology.protein Parasitology Chickens Flagellin Interleukin-1 |
| Zdroj: | Infection and Immunity. 73:2344-2350 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.73.4.2344-2350.2005 |
| Popis: | Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In chickens, the differential biology of Salmonella serovars (systemic versus gut-restricted localization) correlates with the presence or absence of flagella, a known TLR5 agonist. Chicken TLR5 (chTLR5) exhibits conserved sequence and structural similarity with mammalian TLR5 and is expressed in tissues and cell populations of immunological and stromal origin. Exposure of chTLR5 + cells to flagellin induced elevated levels of chicken interleukin-1β (chIL-1β) but little upregulation of chIL-6 mRNA. Consistent with the flagellin-TLR5 hypothesis, an aflagellar Salmonella enterica serovar Typhimurium fliM mutant exhibited an enhanced ability to establish systemic infection. During the early stages of infection, the fliM mutant induced less IL-1β mRNA and polymorphonuclear cell infiltration of the gut. Collectively, the data represent the identification and functional characterization of a nonmammalian TLR5 and indicate a role in restricting the entry of flagellated Salmonella into systemic sites of the chicken. |
| Databáze: | OpenAIRE |
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