Correlation Between Hsp90 Induction Kinetics In Murine Leukemia Cells And The Amount Of Cisplatin Over A Wide Range Of Cytostatic Concentrations
| Autor: | Konstantin Grancharov, Nadejda Spassovska, Angelina I. Russinova, Roumiana Detcheva, A. Kenderov, Kolyo G. Kolev |
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| Rok vydání: | 2002 |
| Předmět: |
Cisplatin
Indirect immunofluorescence Clone (cell biology) Antibodies Monoclonal Induction time Biology medicine.disease Induction kinetics Molecular biology Hsp90 General Biochemistry Genetics and Molecular Biology Kinetics Mice Leukemia Immunology Tumor Cells Cultured medicine biology.protein Animals HSP90 Heat-Shock Proteins Leukemia Erythroblastic Acute Cytotoxicity medicine.drug |
| Zdroj: | Zeitschrift für Naturforschung C. 57:407-412 |
| ISSN: | 1865-7125 0939-5075 |
| DOI: | 10.1515/znc-2002-3-434 |
| Popis: | The induction of HSP90 in murine erythroleukemia cells, clone F4 N, by cisplatin (DDP) was examined using indirect immunofluorescence and avidin-biotin technique, and compared with cisplatin cytotoxicity. A reverse dependence of HSP90 induction time was found on a wide range of cisplatin concentrations (0.5D10 μᴍ), which proved to be cytostatic up to 48 h of continuous treatment. Thus, the observed induction pattern of HSP90 in F4 N cells strictly correlated with their high tolerance toward DDP. This indicates that HSP90 might be responsible, at least in part, for cisplatin resistance of F4 N cells |
| Databáze: | OpenAIRE |
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