Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors
| Autor: | Carmen Klein Herenbrink, Jonatan Fullerton Støier, William Dalseg Reith, Abeer Dagra, Miguel Alejandro Cuadrado Gregorek, Reto B. Cola, Tommaso Patriarchi, Yulong Li, Lin Tian, Ulrik Gether, Freja Herborg |
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| Přispěvatelé: | University of Zurich |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Neurons
Neurotransmitter Agents Dopamine 1.1 Normal biological development and functioning Neurons/metabolism Neurosciences 10050 Institute of Pharmacology and Toxicology Medicine (miscellaneous) 610 Medicine & health Corpus Striatum General Biochemistry Genetics and Molecular Biology Dopamine/metabolism Corpus Striatum/metabolism Underpinning research Neurological 570 Life sciences biology Learning 2.1 Biological and endogenous factors Aetiology General Agricultural and Biological Sciences |
| Zdroj: | Communications Biology, 5 Communications biology, vol 5, iss 1 Klein Herenbrink, C, Støier, J F, Reith, W D, Dagra, A, Gregorek, M A C, Cola, R B, Patriarchi, T, Li, Y, Tian, L, Gether, U & Herborg, F 2022, ' Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors ', Communications Biology, vol. 5, no. 1, 578 . https://doi.org/10.1038/s42003-022-03488-5 |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-022-03488-5 |
| Popis: | Dopamine supports locomotor control and higher brain functions such as motivation and learning. Consistently, dopaminergic dysfunction is involved in a spectrum of neurological and neuropsychiatric diseases. Detailed data on dopamine dynamics is needed to understand how dopamine signals translate into cellular and behavioral responses, and to uncover pathological disturbances in dopamine-related diseases. Genetically encoded fluorescent dopamine sensors have recently enabled unprecedented monitoring of dopamine dynamics in vivo. However, these sensors' utility for in vitro and ex vivo assays remains unexplored. Here, we present a blueprint for making dopamine sniffer cells for multimodal dopamine detection. We generated sniffer cell lines with inducible expression of seven different dopamine sensors and perform a head-to-head comparison of sensor properties to guide users in sensor selection. In proof-of-principle experiments, we apply the sniffer cells to record endogenous dopamine release from cultured neurons and striatal slices, and for determining tissue dopamine content. Furthermore, we use the sniffer cells to measure dopamine uptake and release via the dopamine transporter as a radiotracer free, high-throughput alternative to electrochemical- and radiotracer-based assays. Importantly, the sniffer cell framework can readily be applied to the growing list of genetically encoded fluorescent neurotransmitter sensors. Communications Biology, 5 ISSN:2399-3642 |
| Databáze: | OpenAIRE |
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