Identification of proteins resembling G-protein alpha subunits in locust muscle
| Autor: | A. Soling, Chr. Walther, Walter Rosenthal |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Azides
GTP' G protein Macromolecular Substances Immunoblotting Biophysics Grasshoppers Biology medicine.disease_cause Pertussis toxin Biochemistry GTP-binding protein regulators Adenosine Triphosphate GTP-Binding Proteins medicine Animals Humans Virulence Factors Bordetella Molecular Biology Gene Library Muscles Cholera toxin Skeletal muscle Affinity Labels Cell Biology biology.organism_classification Molecular Weight medicine.anatomical_structure Pertussis Toxin G12/G13 alpha subunits Guanosine Triphosphate Locust |
| Zdroj: | Biochemical and biophysical research communications. 180(2) |
| ISSN: | 0006-291X |
| Popis: | In locust skeletal muscle, FMRFamide-like peptides decrease a K+ conductance. Functional data suggest the involvement of G-proteins. For identification of G-protein alpha-subunits, membranes of locust skeletal muscle were probed with ADP-ribosylating bacterial toxins, the photoreactive GTP analog, [alpha-32P]GTP azidoanilide, and with antibodies against mammalian alpha-subunits. Multiple guanine nucleotide-binding proteins of approximately 24-95 kDa were detected. Pertussis toxin catalyzed the ADP-ribosylation of two proteins comigrating with the ADP-ribosylated alpha-subunits of the mammalian G-proteins Go and Gi. Cholera toxin promoted ADP-ribosylation of a protein comigrating with mammalian cholera toxin substrates (i.e., Gs alpha-subunits). An antibody against mammalian Go alpha-subunits detected a 54-kDa protein. Thus proteins with properties of mammalian G-protein subunits are present in insect muscle. |
| Databáze: | OpenAIRE |
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