Interbase FRET in RNA: from A to Z
| Autor: | Erik Weis, Anders Foller Füchtbauer, Pauline Pfeiffer, Mattias Bood, Jesper R. Nilsson, Anders Dahlén, Marcus Wilhelmsson, Moa Sandberg Wranne, Morten Grøtli |
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| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular 0303 health sciences RNA Conformation Protein Data Bank (RCSB PDB) RNA DNA Biology 010402 general chemistry Ribonucleoside 01 natural sciences Acceptor Fluorescence 0104 chemical sciences 03 medical and health sciences Förster resonance energy transfer Chemical Biology and Nucleic Acid Chemistry Fluorescence Resonance Energy Transfer Genetics Biophysics Nucleic acid Nucleic Acid Conformation Base Pairing 030304 developmental biology |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation. |
| Databáze: | OpenAIRE |
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