Effect of fluoride-substituted apatite on in vivo bone formation
| Autor: | Masahisa Inoue, Hitoshi Nagatsuka, Etsuo Kishimoto, Shin Takagi, Hidetsugu Tsujigiwa, Andrea Paola Rodriguez, Noriyuki Nagai, Racquel Z. LeGeros, Miho Inoue |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Materials science Biomedical Engineering Dentistry chemistry.chemical_element Osteoclasts Calcium Bone tissue Apatite Bone and Bones Biomaterials chemistry.chemical_compound Fluorides In vivo Osteogenesis Apatites Bone cell medicine Animals neoplasms biology Tibia business.industry Magnesium Acid phosphatase Cell Differentiation digestive system diseases Rats medicine.anatomical_structure chemistry visual_art Bone Substitutes biology.protein visual_art.visual_art_medium Biophysics business Fluoride |
| Zdroj: | Journal of biomaterials applications. 25(8) |
| ISSN: | 1530-8022 |
| Popis: | Biological apatites are characterized by the presence of minor constituents such as magnesium (Mg), chloride (Cl), or fluoride (F) ions. These ions affect cell proliferation and osteoblastic differentiation during bone tissue formation. F-substituted apatites are being explored as potential bonegraft materials. The aim of the present study is to investigate the mechanism of bone formation induced by fluoride-substituted apatite (FAp) by analyzing the effect of FAp on the process of in vivo bone formation. FAps containing different F concentrations ( l-FAp: 0.48wt%, m-FAp: 0.91wt%, h-FAp: 2.23wt%) and calcium-deficient apatite (CDA), as positive control, were implanted in rat tibia and bone formation was evaluated by histological examination, immuhistochemistry, in situ hybridization and tartrate-resistant acid phosphatase examinations. The results showed that l-FAp, m-FAp, h-FAp, and CDA biomaterials allowed migration of macrophages, attachment, proliferation, and phenotypic expression of bone cells leading to new bone formation in direct apposition to the particles. However, the l-FAp preparation allowed faster bone conduction compared to the other experimental materials. These results suggest that FAp with low F concentration may be an efficient bonegraft material for dental and medical application. |
| Databáze: | OpenAIRE |
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