Normal and Leukaemic Human Haemopoietic Cells in Diffusion Chamber
| Autor: | Geneviève Clemancey-Marcille, Daniel Hollard, Monique Laurent |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Myeloid Adolescent Cell division Population Bone Marrow Cells Cell Count Biology Lymphocyte Activation Colony-Forming Units Assay Mice medicine Animals Humans Lymphocytes Progenitor cell education Cells Cultured Aged Diminution education.field_of_study Blood Cells Macrophages Hematology Middle Aged Hematopoietic Stem Cells medicine.disease Molecular biology Hematopoiesis Leukemia Myeloid Acute Leukemia Haematopoiesis medicine.anatomical_structure Female Myelocyte Cell Division Granulocytes |
| Zdroj: | Scandinavian Journal of Haematology. 24:205-212 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1980.tb01328.x |
| Popis: | Leukaemic human bone marrow and peripheral blood cells were cultured for 25 d in diffusion chambers implanted into cyclophosphamide treated mice. Normal bone marrow cells were cultured simultaneously. These cells were studied both morphologically and functionally (CFU-C). The leukaemic cells behaved heterogeneously, 2 groups being distinguishable in accordance with their initial in vitro growth pattern (1: no growth or microcluster growth. 2: macrocluster growth). Group I showed progressive cellular death with a diminution of granulocytic progenitors and the appearance of a predominantly macrophagic population. This behaviour resembled that of the control group. The initial microcluster growth pattern remained identical throughout the entire culture period. Group 2, after considerable cellular death up to d 5, showed an explosive proliferation of the granulocytic progenitors and incomplete differentiation (up to myelocyte). The initial macrocluster growth pattern remained identical. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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