A small group of neurosecretory cells expressing the transcriptional regulator apontic and the neuropeptide corazonin mediate ethanol sedation in Drosophila
| Autor: | Ulrike Heberlein, Kimberly D. McClure |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Nervous system
medicine.medical_specialty Sedation Green Fluorescent Proteins Neuropeptide Biology Animals Genetically Modified Internal medicine medicine Transcriptional regulation Gene silencing Animals Drosophila Proteins Hypnotics and Sedatives RNA Messenger Regulation of gene expression Neurons Behavior Animal Dose-Response Relationship Drug Ethanol General Neuroscience Neuropeptides Metamorphosis Biological Brain Articles biology.organism_classification Neurosecretory Systems Cell biology DNA-Binding Proteins Corazonin Endocrinology medicine.anatomical_structure Gene Expression Regulation Mutation Drosophila medicine.symptom Drosophila melanogaster Transcription Factors |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(9) |
| ISSN: | 1529-2401 |
| Popis: | In the fruit flyDrosophila melanogaster, as in mammals, acute exposure to a high dose of ethanol leads to stereotypical behavioral changes beginning with increased activity, followed by incoordination, loss of postural control, and eventually, sedation. The mechanism(s) by which ethanol impacts the CNS leading to ethanol-induced sedation and the genes required for normal sedation sensitivity remain largely unknown. Here we identify the geneapontic(apt), an Myb/SANT-containing transcription factor that is required in the nervous system for normal sensitivity to ethanol sedation. Using genetic and behavioral analyses, we show thataptmediates sensitivity to ethanol sedation by acting in a small set of neurons that express Corazonin (Crz), a neuropeptide likely involved in the physiological response to stress. The activity of Crz neurons regulates the behavioral response to ethanol, as silencing and activating these neurons affects sedation sensitivity in opposite ways. Furthermore, this effect is mediated by Crz, as flies with reducedcrzexpression show reduced sensitivity to ethanol sedation. Finally, we find that bothaptandcrzare rapidly upregulated by acute ethanol exposure. Thus, we have identified two genes and a small set of peptidergic neurons that regulate sensitivity to ethanol-induced sedation. We propose that Apt regulates the activity of Crz neurons and/or release of the neuropeptide during ethanol exposure. |
| Databáze: | OpenAIRE |
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