Expression of Matrix Metalloproteinases (MMP)-12 by Myofibroblasts during Alkali-Burned Corneal Wound Healing
| Autor: | Hiroshi Takahashi, Masamichi Ishizaki, Haruhi Iwanami, Yuh Fukuda |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty In situ hybridization Matrix metalloproteinase Gene Expression Regulation Enzymologic Cornea Immunoenzyme Techniques Cellular and Molecular Neuroscience Matrix Metalloproteinase 12 Burns Chemical medicine Animals Sodium Hydroxide RNA Messenger Fluorescent Antibody Technique Indirect In Situ Hybridization Regulation of gene expression Wound Healing Messenger RNA Arc (protein) Reverse Transcriptase Polymerase Chain Reaction Chemistry Fibroblasts Molecular biology Actins Sensory Systems Extracellular Matrix Eye Burns Ophthalmology Immunohistochemistry Rabbits sense organs Wound healing Myofibroblast |
| Zdroj: | Current Eye Research. 34:207-214 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1080/02713680802687809 |
| Popis: | Purpose: The purpose of this study was to determine the expression of MMP-12 by myofibroblasts during the healing of alkali-burned rabbit corneas (ARC), thus implicating its role in ECM remodeling. Methods: Rabbit corneas during alkali burn were examined for MMP-12 mRNA expression by RT-PCR. Immunohistochemistry was used to determine the presence of α-SMA, MMP-12 protein, and macrophages. In situ hybridization was performed to identify MMP-12 mRNA expressing cells. Results: RT-PCR showed that MMP-12 mRNA was expressed in the alkali-burned corneas from one week after the injury. Immunohistochemistry showed myofibroblasts positive for MMP-12 expression. In situ hybridization revealed that MMP-12 mRNA was expressed by myofibroblasts. Conclusion: Our results indicate that, in alkali-burned corneas, myofibroblasts express both MMP-12 mRNA and protein. We suggest that MMP-12 may disintegrate some components of the ECM released after severe alkali burn, which may be involved in the ECM remodeling. |
| Databáze: | OpenAIRE |
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