Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Autor: Agnieszka Szewczyk, Marek Szlósarczyk, Ricardo Nunes, Pawel Pasko, Małgorzata Tyszka-Czochara, Isabel S. Carvalho
Rok vydání: 2016
Předmět:
Gallic acid
Protocatechuic acid
Pharmaceutical Science
Crataegus-monogyna
Ferric Compounds
01 natural sciences
Antioxidants
Essential oil
In-vitro
Drug Discovery
Chromatography
High Pressure Liquid
Chromatography
Reverse-Phase
biology
Traditional medicine
Mentha suaveolens
Liver Neoplasms
solvent influence
Hep G2 Cells
General Medicine
Geranium purpureum
Antimicrobial
Anti-Bacterial Agents
Zinc
Molecular Medicine
Lavandula-luisieri
Antibacterial activity
Oxidation-Reduction
Geraniaceae
Lavandula stoechas
Phenolic-compounds
Research Article
Antimicrobial activities
Equisetum
phenolics
Microbial Sensitivity Tests
hplc
traditional medicine
Enterococcus faecalis
Lead-induced cytotoxicity
food
Chlorides
Phenols
Botany
Humans
Cell Proliferation
algarve
Pharmacology
Plants
Medicinal
Bacteria
Dose-Response Relationship
Drug
Portugal
Plant Extracts
010405 organic chemistry
lcsh:RM1-950
Plant Components
Aerial
biology.organism_classification
Antineoplastic Agents
Phytogenic
food.food
0104 chemical sciences
010404 medicinal & biomolecular chemistry
lcsh:Therapeutics. Pharmacology
Complementary and alternative medicine
Pathogenic bacteria
flavonoids
Solvents
Lamiaceae
Phytotherapy
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Pharmaceutical Biology, Vol 55, Iss 1, Pp 114-123 (2017)
Pharmaceutical Biology
Scopus-Elsevier
ISSN: 1744-5116
1388-0209
Popis: Context: Crataegus monogyna L. (Rosaceae) (CM), Equisetum telmateia L. (Equisataceae) (ET), Geranium purpureum Vil. (Geraniaceae) (GP), Mentha suaveolens Ehrh. (Lamiaceae) (MS), and Lavandula stoechas L. spp. luisieri (Lamiaceae) (LS) are all medicinal. Objective: To evaluate the antioxidant, antiproliferative and antimicrobial activities of plant extracts and quantify individual phenolics and zinc. Material and methods: Aerial part extracts were prepared with water (W), ethanol (E) and an 80% mixture (80EW). Antioxidant activity was measured with TAA, FRAP and RP methods. Phenolics were quantified with a HPLC. Zinc was quantified using voltammetry. Antibacterial activity (after 48 h) was tested using Enterococcus faecalis, Bacillus cereus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes. Antiproliferative activity (after 24 h) was tested using HEP G2 cells and fibroblasts. Results: Solvents influenced results; the best were E and 80EW. GP had the highest antioxidant activity (TAA and FRAP of 536.90mg AAE/g dw and 783.48mg TE/g dw, respectively). CM had the highest zinc concentration (37.21 mg/kg) and phenolic variety, with neochlorogenic acid as the most abundant (92.91 mg/100 g dw). LS was rich in rosmarinic acid (301.71 mg/100 g dw). GP and LS inhibited the most microorganisms: B. cereus, E. coli and S. aureus. GP also inhibited E. faecalis. CM had the lowest MIC: 5830 mu g/mL. The antibacterial activity is explained by the phenolics present. LS and CM showed the most significant anti-proliferative activity, which is explained by their zinc content. Conclusion: The most promising plants for further studies are CM, LS and GP. FCT, Fundacao para a Ciencia e a Tecnologia of Portugal [SFRH/BSA/139/2014]
Databáze: OpenAIRE
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