Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola
| Autor: | Helio Langoni, Lídia Raquel de Carvalho, Paulo Francisco Domingues, Aristeu Vieira da Silva, Veruska Maia da Costa, Rodrigo Costa da Silva, Fabio Hiroto Shimabukuro |
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| Přispěvatelé: | Instituto Adolfo Lutz (IAL), Universidade Estadual Paulista (Unesp), Universidade Estadual de Feira de Santana (UEFS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 lcsh:QR1-502 Enzyme-Linked Immunosorbent Assay Leptospira interrogans serovar canicola lcsh:Microbiology Serology Microbiology microscopic agglutination test Mice Direct agglutination test Agglutination Tests medicine Animals Humans leptospirosis biology Brucellosis medicine.disease Virology Leptospirosis Antibodies Bacterial Agglutination (biology) biology.protein Syphilis Female prozone effect Antibody Technical Notes |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 5, Pages: 668-670, Published: AUG 2013 SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 5, Pp 668-670 (2013) Memórias do Instituto Oswaldo Cruz |
| ISSN: | 0074-0276 |
| Popis: | Made available in DSpace on 2014-10-01T13:08:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-08-01Bitstream added on 2014-10-01T14:03:00Z : No. of bitstreams: 1 S0074-02762013000500668.pdf: 325974 bytes, checksum: 07c5116e68c99a1aa5133336063feda6 (MD5) Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs. Instituto Adolfo Lutz Centro de Laboratorio Regional XI Universidade Estadual Paulista Faculdade de Medicina Veterinaria e Zootecnia Departamento de Higiene Veterinaria e Saude Publica Universidade Estadual de Feira de Santana Departamento de Ciencias Biologicas Universidade Estadual Paulista Instituto de Biociencias Departamento de Bioestatistica Universidade Estadual Paulista Faculdade de Medicina Veterinaria e Zootecnia Departamento de Higiene Veterinaria e Saude Publica Universidade Estadual Paulista Instituto de Biociencias Departamento de Bioestatistica |
| Databáze: | OpenAIRE |
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