Leptin Receptors and β2-Adrenergic Receptor mRNA Expression in Brain Injury-Related Heterotopic Ossification
| Autor: | Odile Broux, Joseph Jeanfils, Pierre Hardouin, Jean-Christophe Devedjian, Christophe Delecourt, Christophe Chauveau |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Leptin Male Gene isoform medicine.medical_specialty Gene Expression Biology Indirect pathway of movement Osteocytes Biochemistry Internal medicine Gene expression medicine Humans RNA Messenger Receptor Molecular Biology Leptin receptor Ossification Ossification Heterotopic Cell Biology Middle Aged medicine.disease Endocrinology Brain Injuries Receptors Leptin Female Heterotopic ossification Receptors Adrenergic beta-2 medicine.symptom Proto-Oncogene Proteins c-fos |
| Zdroj: | Journal of Receptors and Signal Transduction. 28:347-359 |
| ISSN: | 1532-4281 1079-9893 |
| DOI: | 10.1080/10799890802239762 |
| Popis: | Heterotopic ossification (HO) frequently occurs after brain injury. Recently, we found that leptin levels were decreased in the serum of patients with HO. Data suggest two mechanisms mediating leptin effects: a central suppressive mechanism acting via the beta(2)-adrenergic system and a direct stimulatory action starting when leptin binds to its receptors in osteoblastic cells. In this study, we analyzed leptin and beta(2)-adrenergic receptors mRNA expression in osteocytes originated from normal or heterotopic bone biopsies to investigate whether direct or indirect pathway signaling might be implicated in this pathological bone formation. We report for the first time the mRNA expression of the leptin receptor isoforms in osteocytes isolated from all biopsies. Moreover, quantitative reverse transcription-polymerase chain reaction allowed us to measure a significant decrease in the level of beta(2)-adrenergic receptor mRNA in cells isolated from heterotopic bone biopsies. These results could suggest an association between hypothalamic leptin signaling and brain injury-related HO. |
| Databáze: | OpenAIRE |
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