The Fate of Thymocytes Labeledin Vivowith CFSE
| Autor: | Edouard F. Potworowski, Claire Beauchemin, Yves St-Pierre, Monica Graziano, Marcel Desrosiers |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
CD4-Positive T-Lymphocytes
CD3 Complex Microinjections Fluorescent Antibody Technique Succinimides Spleen Thymus Gland CD8-Positive T-Lymphocytes Biology Sensitivity and Specificity Mice In vivo medicine Animals Fluorescent Dyes Skin Cell Biology Fluoresceins In vitro Cell biology Mice Inbred C57BL Kinetics Thymocyte Lymphatic system medicine.anatomical_structure Toxicity Immunology Immunohistochemistry Lymph Nodes Lymph Biomarkers Cell Division |
| Zdroj: | Experimental Cell Research. 240:75-85 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.1997.3900 |
| Popis: | The fate of thymic emigrants had so far been studied using a variety of markers, each of which had inherent limitations as to stability, toxicity, or selectivity. We describe a new technique which relies on the in vivo injection of CFSE, an esterified vital dye hitherto used at 80 times lower concentrations for in vitro cell labeling. We show that CFSE labels a representative sample of all thymocyte subsets and that these migrate at a rate of approximately 2–3 × 10 6 cells/day to peripheral lymphoid organs. We show that they enter lymph nodes at day 1 postinjection and stay for at least 21 days, whereas the turnover in the spleen is more rapid. We also show by immunohistochemistry, using peroxidase-labeled anti-FITC antibodies, that CFSE-labeled thymic emigrants are confined to T-dependent areas of peripheral lymphoid organs. |
| Databáze: | OpenAIRE |
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