Evaluation of laboratory methods routinely used to detect the effect of aspirin against new reference methods
| Autor: | E. Kovács, Eszter Tóth, Éva Katona, István Édes, László Balogh, Róbert Gábor Kiss, László Muszbek, Nóra Homoródi, Zsuzsanna Bereczky, Hajna Péterfy |
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| Rok vydání: | 2014 |
| Předmět: |
Agonist
Adult Blood Platelets Male medicine.drug_class Thromboxane aspirin platelet secretion Pharmacology Thromboxane A2 chemistry.chemical_compound Young Adult Reference Values medicine Humans Platelet Elméleti orvostudományok Aspirin Dose-Response Relationship Drug Chemistry Orvostudományok Hematology Middle Aged Laboratories Hospital Thromboxane B2 Epinephrine aspirin resistance platelet aggregation reference method Platelet-rich plasma Immunology Cyclooxygenase 1 Female Platelet Aggregation Inhibitors medicine.drug thromboxane |
| Zdroj: | Thrombosis Research. 133(5):811-816 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/j.thromres.2013.10.008 |
| Popis: | Background Aspirin, a commonly used antiplatelet agent, blocks platelet thromboxane A 2 (TXA 2 ) formation from arachidonic acid (AA) by acetylating platelet cyclooxygenase-1 (COX-1). Laboratory methods currently used to detect this antiplatelet effect of aspirin provide variable results. We have reported three methods that assess platelet COX-1 acetylation (inactivation) by aspirin and its direct consequences. The first and second assays use monoclonal anti-human-COX-1 antibodies that only detect acetylated (inactivated) COX-1 and active (non-acetylated) COX-1, respectively. The third method measures platelet production of TXB 2 (the stable metabolite of TXA 2 ) in vitro in response to AA. We compared the results of these three reference methods with other routinely used methods for assessing the functional consequences aspirin treatment. Methods 108 healthy volunteers were treated with low-dose aspirin for 7days. On day 7 following aspirin treatment COX-1 in the platelets was fully acetylated whereas only non-acetylated COX-1 was present in the day 0 platelets. Further, TXB 2 production by day 7 platelets was completely blocked. The following tests were performed on the samples obtained from study participants before and after seven days of aspirin treatment: PFA-100 closure time with collagen/epinephrine cartridge, VerifyNow® (VN) Aspirin Assay, platelet aggregation and ATP secretion using AA, ADP, epinephrine and collagen as agonists. Results Comparing the pre-treatment and day 7 values, methods that use AA as platelet agonist (AA-induced platelet aggregation/secretion and VN Aspirin Assay) showed high discriminative power. In contrast, results of the other tests showed considerable overlap between day 7 and day 0 values. Conclusions Only assays that clearly distinguish between acetylated and non-acetylated platelet COX-1 are useful for establishing the antiplatelet effect of aspirin. The other tests are not suitable for this purpose. |
| Databáze: | OpenAIRE |
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