Alterations of K-ras and p53 mutations in colorectal cancer patients in Central Europe
| Autor: | Agota Kovacs, Angela Rasinariu, Ken Soyonki, Barry W. Glickman, Wolfgang C. Kusser, András Jeney, Zoltan L. Marcsek, Anghel R. Popp, Edwin A. König, Istvan Köves |
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| Rok vydání: | 2001 |
| Předmět: |
Tumor suppressor gene
Colorectal cancer Health Toxicology and Mutagenesis Biology Toxicology Malignancy Proto-Oncogene Mas law.invention Exon law medicine Humans Gene Polymerase chain reaction Polymorphism Single-Stranded Conformational DNA Primers Genetics Reverse Transcriptase Polymerase Chain Reaction Point mutation Single-strand conformation polymorphism DNA Neoplasm Exons Sequence Analysis DNA medicine.disease Introns Europe Genes ras Cancer research Tumor Suppressor Protein p53 Colorectal Neoplasms |
| Zdroj: | Journal of toxicology and environmental health. Part A. 62(5) |
| ISSN: | 1528-7394 |
| Popis: | Many molecular investigations of colorectal cancer (CRC) have suggested that the accumulation of specific mutations in proto-oncogenes and tumor suppressor genes regulating cell growth via signal transduction trigger the stagewise progression to malignancy. In this study, the frequency, location, and type of mutations of the K-ras proto-oncogene exon I and p53 tumor suppressor gene exons 5-8 were analyzed in colorectal carcinomas of 65 patients from Central Europe, using polymerase chain reaction (PCR)-cold single-strand conformation polymorphism (SSCP) screening and direct sequencing. The incidence of K-ras activating mutations in these Central European samples was lower (25%) compared to that obtained in American and western European populations (40-50% at least), while the incidence of p53 inactivating mutations was similar (58%). These results suggest that some other genetically linked mechanisms may play a role in CRC development and progression, and hence K-ras and p53 mutations cannot be considered to be universal genetic markers for CRC. |
| Databáze: | OpenAIRE |
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