A Major Locus Controls a Genital Shape Difference Involved in Reproductive Isolation Between Drosophila yakuba and Drosophila santomea
| Autor: | Rosina Savisaar, Virginie Orgogozo, David L. Stern, Vincent Debat, Alexandre E. Peluffo, Isabelle Nuez |
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| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Janelia Farm Research Campus, Howard Hughes Medical Institute (HHMI), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Biologie Intégrative des Populations, École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Howard Hugues Medical Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetic Markers
Male Reproductive Isolation Genotype QTL Quantitative Trait Loci Locus (genetics) Quantitative trait locus Investigations shape Species Specificity Genetics Animals lock-and-key Molecular Biology Genetics (clinical) Crosses Genetic Genetic Association Studies Morphometrics [SDV.GEN]Life Sciences [q-bio]/Genetics biology Models Genetic Chromosome Mapping genitalia Reproductive isolation biology.organism_classification Biological Evolution Genetic architecture Chromosomes Insect Phenotype Evolutionary biology Genetic marker Female Drosophila Drosophila melanogaster [SDE.BE]Environmental Sciences/Biodiversity and Ecology Drosophila yakuba |
| Zdroj: | G3 G3, 2015, 5 (12), pp.2893-2901. ⟨10.1534/g3.115.023481⟩ G3, Genetics Society of America, 2015, 5 (12), pp.2893-2901. ⟨10.1534/g3.115.023481⟩ G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.115.023481⟩ |
| Popis: | Rapid evolution of genitalia shape, a widespread phenomenon in animals with internal fertilization, offers the opportunity to dissect the genetic architecture of morphological evolution linked to sexual selection and speciation. Most quantitative trait loci (QTL) mapping studies of genitalia divergence have focused on Drosophila melanogaster and its three most closely related species, D. simulans, D. mauritiana, and D. sechellia, and have suggested that the genetic basis of genitalia evolution involves many loci. We report the first genetic study of male genitalia evolution between D. yakuba and D. santomea, two species of the D. melanogaster species subgroup. We focus on male ventral branches, which harm females during interspecific copulation. Using landmark-based geometric morphometrics, we characterized shape variation in parental species, F1 hybrids, and backcross progeny and show that the main axis of shape variation within the backcross population matches the interspecific variation between parental species. For genotyping, we developed a new molecular method to perform multiplexed shotgun genotyping (MSG), which allowed us to prepare genomic DNA libraries from 365 backcross individuals in a few days using little DNA. We detected only three QTL, one of which spans 2.7 Mb and exhibits a highly significant effect on shape variation that can be linked to the harmfulness of the ventral branches. We conclude that the genetic architecture of genitalia morphology divergence may not always be as complex as suggested by previous studies. |
| Databáze: | OpenAIRE |
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