Potential protection of curcumin against amyloid β-induced toxicity on cultured rat prefrontal cortical neurons
| Autor: | Jia Cui, Xiao-Yan Qin, Long-Chuan Yu, Yong Cheng, Yan Zhang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Curcumin
Amyloid beta Prefrontal Cortex Apoptosis Caspase 3 Pharmacology Rats Sprague-Dawley chemistry.chemical_compound Western blot mental disorders medicine Animals Cells Cultured Neurons Amyloid beta-Peptides TUNEL assay biology medicine.diagnostic_test General Neuroscience Molecular biology Peptide Fragments Rats Neuroprotective Agents medicine.anatomical_structure Animals Newborn Proto-Oncogene Proteins c-bcl-2 chemistry Toxicity biology.protein Neuron |
| Zdroj: | Neuroscience Letters. 463:158-161 |
| ISSN: | 0304-3940 |
| Popis: | The present study explored the effect of curcumin against amyloid beta (Abeta)-induced toxicity on cultured rat primary prefrontal cortical neurons. The results showed that administration of 10 microM of curcumin induced significantly protection against 20 microM of Abeta(25-35)-induced toxicity on the cultured rat primary prefrontal cortical neurons tested by MTT and TUNEL assays. We further examined the involvements of the apoptotic or anti-apoptotic proteins in curcumin protection against Abeta(25-35)-induced cytotoxicity on cultured neurons and found that the content of apoptotic protein caspase-3 was increased and the content of anti-apoptotic factor Bcl2 was decreased significantly after Abeta(25-35) treatments, while administration of curcumin significantly inhibited the Abeta(25-35)-induced increases in the content of caspase-3 and inhibited the Abeta(25-35)-induced decreases in the content of Bcl2 tested by Western blot. The results suggest that curcumin protects cultured rat primary prefrontal cortical neurons against Abeta-induced cytotoxicity, and both Bcl2 and caspase-3 are involved in the curcumin-induced protective effects. |
| Databáze: | OpenAIRE |
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