Characterization of the Bovineκ-Casein Gene Promoter
| Autor: | Naohito Aoki, Jung-Youb Ahn, Takahiro Adachi, Tsukasa Matsuda, Ryo Nakamura, Kazue Yamamoto |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
TATA box
Molecular Sequence Data Biology Transfection medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Biochemistry DNA-binding protein Cell Line Analytical Chemistry Mice Gene expression medicine Animals Promoter Regions Genetic Molecular Biology Gene Mutation Base Sequence Organic Chemistry Caseins Promoter General Medicine TATA Box Molecular biology DNA-Binding Proteins Regulatory sequence Cattle Plasmids Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 60:1937-1940 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.60.1937 |
| Popis: | kappa-Casein gene promoter was localized within a 570-bp fragment (-552/+18) of a 5'-flanking region by the gene transfection assay. Deletion mutation analysis in mammary epithelial cell line, HC11, suggested that there are regulatory element in a region from -439 through -125. Some nuclear proteins from lactating rate mammary gland bind to this region specifically. One of them expressed preferentially during pregnancy bound to a 132-bp fragment (-439/-308) and another expressed preferentially during lactation bound to a 183-bp fragment (-307/-125). |
| Databáze: | OpenAIRE |
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