Hyaluronic acid conjugated superparamagnetic iron oxide nanoparticle for cancer diagnosis and hyperthermia therapy
| Autor: | Hyegyeong Lee, Reju George Thomas, Myeong Ju Moon, Arathyram Ramachandra Kurup Sasikala, In-Kyu Park, Cheol Sang Kim, Yong Yeon Jeong |
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| Rok vydání: | 2015 |
| Předmět: |
Hyperthermia
Male Pathology medicine.medical_specialty Time Factors Polymers and Plastics Biocompatibility medicine.medical_treatment Mice Nude chemistry.chemical_compound Mice Cell Line Tumor Neoplasms Hyaluronic acid PEG ratio Materials Chemistry medicine Animals Humans Viability assay Hyaluronic Acid Magnetite Nanoparticles Mice Inbred BALB C biology Cell Death Staining and Labeling Chemistry Organic Chemistry CD44 Temperature Dextrans Hyperthermia Induced medicine.disease Magnetic Resonance Imaging Hyperthermia therapy Endocytosis MCF-7 biology.protein NIH 3T3 Cells Biomedical engineering |
| Zdroj: | Carbohydrate polymers. 131 |
| ISSN: | 1879-1344 |
| Popis: | Recently, superparamagnetic iron oxide nanoparticles (SPIONs) have been prepared for magnetic resonance (MR) imaging and hyperthermia therapy. Here, we have developed hyaluronic acid (HA) coated SPIONs primarily for use in a hyperthermia application with an MR diagnostic feature with hydrodynamic size measurement of 176nm for HA-PEG10-SPIONs and 149nm for HA-SPIONs. HA-coated SPIONs (HA-SPIONs) were prepared to target CD44-expressed cancer where the carrier was conjugated to PEG for analyzing longer circulation in blood as well as for biocompatibility (HA-PEG10 SPIONs). Characterization was conducted with TEM (shape), DLS (size), ELS (surface charge), TGA (content of polymer) and MRI (T2-relaxation time). The heating ability of both the HA-SPIONs and HA-PEG10-SPIONs was studied by AMF and SAR calculation. Cellular level tests were conducted using SCC7 and NIH3T3 cell lines to confirm cell viability and cell specific uptake. HA-SPIONs and HA-PEG10-SPIONs were injected to xenograft mice bearing the SCC7 cell line for MRI cancer diagnosis. We found that HA-SPION-injected mice tumors showed nearly 40% MR T2 contrast compared to the 20% MR T2 contrast of the HA-PEG10-SPION group over a 3h time period. Finally, in vitro hyperthermia studies were conducted in the SCC7 cell line that showed less than 40% cell viability for both HA-SPIONs and HA-PEG10-SPIONs in AMF treated cells. In conclusion, HA-SPIONs were targeted specifically to the CD44, and the hyperthermia effect of HA-SPIONs and HA-PEG10-SPIONs was found to be significant for future studies. |
| Databáze: | OpenAIRE |
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