Vsebnost ekstraktivov v skorji in lesu robinije (Robinia pseudoacacia L.)
| Autor: | Ida Poljanšek, Blaž Vivod, Primož Oven, Viljem Vek |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Acta Silvae et Ligni, vol. 119, pp. 13-25, 2019. Acta Silvae et Ligni, Vol 119, Pp 13-25 (2019) |
| ISSN: | 2335-3953 2335-3112 |
| DOI: | 10.20315/asetl.119.2 |
| Popis: | Raziskali smo vsebnost in sestavo ekstraktivov v lesnih tkivih in skorji robinije (Robinia pseudoacacia L.). Vzorce njenega lesa in skorje smo ekstrahirali v aparatu Soxhlet z acetonom. Acetonske ekstrakte smo preiskali gravimetrično in s tem izmerili vsebnost skupnih hidrofilnih ekstraktivov. Z UV-Vis spektrofotometrom smo kolorimetrično izmerili vsebnost skupnih fenolov. Ekstrakte lesa in skorje robinije smo nato detajlno preiskali s sistemom za tekočinsko kromatografijo visoke ločljivosti (HPLC). S to tehniko smo v ekstraktih identificirali in kvantitativno ovrednotili dihidrorobinetin. Največje vsebnosti skupnih hidrofilnih ekstraktivov, skupnih fenolov in dihidrorobinetina smo izmerili v vzorcih jedrovine. Značilno manj ekstraktivov je bilo v vzorcih beljave in skorje. Aksialne variabilnosti v vsebnosti topnih komponent za les in skorjo robinije nismo dognali. Ugotovili smo, da je jedrovina robinije bogat vir fenolnih ekstraktivov. The content and composition of extractives in the wood and bark tissue of black locust (Robinia pseudoacacia L.) were investigated. Wood and bark samples were extracted using a Soxhlet apparatus with acetone. The extracts were gravimetrically examined to measure total hydrophilic extractive content. Total phenolic content was measured colorimetrically with a UV-Vis spectrophotometer. The wood and bark extracts were further investigated using high-pressure liquid chromatography (HPLC) to identify and quantify dihydrorobinetin in the extracts. The contents of total hydrophilic extractives, total phenols and dihydrorobinetin were higher in heartwood than in the sapwood and bark. No relationship was found between the height at which the samples were taken from the stem and the content of soluble components in the wood and bark. The heartwood of black locust represents a rich potential source of phenolic extractives. |
| Databáze: | OpenAIRE |
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