Interaction of human osteoblast-like Saos-2 cells with stainless steel coated by silicalite-1 films
| Autor: | Milan Bousa, Helena Dragounova, Lucie Bacakova, Ivan Jirka, Jan Plšek, Libor Brabec, Marta Vandrovcová |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Biocompatibility Surface Properties Silicon dioxide Scanning electron microscope Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Biomaterials Contact angle chemistry.chemical_compound X-ray photoelectron spectroscopy Cell Adhesion medicine Humans Saos-2 cells Chemical composition Osteoblasts Photoelectron Spectroscopy Osteoblast Silicon Dioxide Stainless Steel 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure chemistry Chemical engineering Mechanics of Materials Microscopy Electron Scanning 0210 nano-technology |
| Zdroj: | Materials Science and Engineering: C. 76:775-781 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2017.03.067 |
| Popis: | This paper investigates the interaction of human osteoblast-like Saos-2 cells with stainless steel covered by a film of densely inter-grown silicalite-1 crystals with defined outer and inner surfaces. The chemical composition of this film, labeled as SF(RT), was tuned by heat treatment at 300°C and 500°C (labeled as SF(300) and SF(500), respectively) and characterized by X-ray photoelectron spectroscopy (XPS), water drop contact angle (WCA) measurements and scanning electron microscopy (SEM). The number, the spreading area and the activity of alkaline phosphatase of human osteoblast-like Saos-2 cells in cultures on the silicalite-1 film were affected by the chemical composition of its outer surface and by its micro-porous structure. The number and the spreading area of the adhered osteoblast-like cells on day 1 was highest on the surface of SF(RT) relative to their adhesion and spreading on a glass cover slip due to the SF(RT) topology. However, SF(300) markedly supported cell growth during days 3 and 7 after seeding. |
| Databáze: | OpenAIRE |
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