In vitro method for prediction of the phototoxic potentials of fluoroquinolones
| Autor: | Y Tsurumaki, M Takei, Y Oomori, M Hosaka, T Yamamoto |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Erythrocytes
Cell Survival Ultraviolet Rays Biology Pharmacology Toxicology Hemolysis Tosufloxacin medicine Enoxacin Humans heterocyclic compounds Antibacterial agent Dose-Response Relationship Drug General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Gatifloxacin Sparfloxacin Lomefloxacin Ofloxacin Phototoxicity DNA Damage Dermatitis Phototoxic Fluoroquinolones HeLa Cells medicine.drug |
| Zdroj: | Toxicology in Vitro. 15:721-727 |
| ISSN: | 0887-2333 |
| Popis: | The phototoxic potential of eight fluoroquinolones (norfloxacin, ofloxacin, enoxacin, ciprofloxacin, lomefloxacin, tosufloxacin, sparfloxacin and gatifloxacin) was evaluated by using three in vitro methods of cytotoxicity against mammalian cells, erythrocyte lysis and DNA strand breakage. All fluoroquinolones tested with the exception of gatifloxacin, an 8-methoxy quinolone, showed DNA strand breaking activities under UV-A irradiation. Their cytotoxicity against HeLa cells was also enhanced by UV-A irradiation. In particular, the phototoxic potential of sparfloxacin, enoxacin and lomefloxacin was high in both methods. Ofloxacin is very photocytotoxic against HeLa cells, while it has low potential to cause DNA strand breakage. Norfloxacin, ciprofloxacin and enoxacin were very photohemolytic, but sparfloxacin was not, indicating that the in vivo phototoxic potencies of fluoroquinolones might not be predictable by the photohemolysis study. Gatifloxacin, a non-phototoxic quinolone, showed no phototoxic potential in any of these three in vitro tests. These results suggest that determination of DNA strand breaking activity, combined with cytotoxicity against mammalian cells, is available to predict the phototoxic potential of fluoroquinolones without laboratory animals. |
| Databáze: | OpenAIRE |
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