Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis
| Autor: | Pei Ming Yang, Sz Hsien Yu, Shu Jun Chiu, Yi Chu Yu, Chih Wen Peng |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Flavonoids
Cancer Research Gene knockdown TUNEL assay Flavonols Poly ADP ribose polymerase Apoptosis HCT116 Cells Molecular biology digestive system diseases Neoplasm Proteins Securin chemistry.chemical_compound Oncology chemistry Annexin Colonic Neoplasms Cancer research Humans Tumor Suppressor Protein p53 Cytotoxicity Fisetin |
| Zdroj: | Cancer Letters. 300:96-104 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2010.09.015 |
| Popis: | Securin is highly-expressed in various tumors including those of the colon. In this study, the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated. Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of procaspase-3 and PARP. These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA, but attenuated when wild-type or non-degradable securin was reconstituted. Moreover, fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells. Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis. Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis. |
| Databáze: | OpenAIRE |
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