Panax ginseng callus, suspension, and root cultures: extraction and qualitative analysis
| Autor: | Svetlana Ivanova, Yuliya Golubtsova, Lev A. Ostroumov, Lyubov Dyshlyuk, Anastasia Igorevna Dmitrieva |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Chromatography plant extracts lcsh:TP368-456 010405 organic chemistry Chemistry gerontology Extraction (chemistry) panax ginseng food and beverages ginseng 01 natural sciences geroprotective properties 0104 chemical sciences 03 medical and health sciences Ginseng lcsh:Food processing and manufacture Qualitative analysis Callus Suspension (vehicle) plant cultures 030304 developmental biology Food Science |
| Zdroj: | Foods and Raw Materials, Vol 8, Iss 2, Pp 369-376 (2020) |
| ISSN: | 2310-9599 2308-4057 |
| Popis: | Introduction. In recent years, scientists have been actively searching for medicinal plants containing biologically active substances with geroprotective properties to treat diseases of old age, in particular cancer, diabetes, cardiovascular diseases, and others. Ginseng (Panax ginseng L.) is a promising source of geroprotective compounds. We aimed to select optimal parameters for extracting organic compounds from ginseng callus, suspension, and root cultures and analyze their qualitative composition. Study objects and methods. We studied ginseng callus, suspension, and root cultures, as well as their extracts. Biologically active substances were extracted with 30 to 70% ethanol. Organic compounds were determined by thin-layer chromatography. The results for each plant were archived and analyzed for the presence of quercetin, mangiferin, luteolin, rutin, quercetin-2-D-glucoside, malvidin, as well as caffeic, cinnamic, ferulic, and sinapinic acids. Results and discussion. We developed a procedure for screening solvents and performed a fractional qualitative analysis of biologically active substances extracted from ginseng. As a result, we established the optimal parameters for extracting biologically active substances from the dried biomass of ginseng cultures. In all cases, temperature and the ratio of solvent to biomass were the same (50°C, 1:5). However, the extraction time and ethanol concentration differed, amounting to 60 min and 50% for callus cultures, 30 min and 60% for suspension cultures, and 60 min and 70% for root cultures. The qualitative analysis of organic compounds showed the presence of rutin (0.25), quercetin (0.75), and mangiferin (0.57), as well as caffeic and sinapinic acids in the extracts. Conclusion. Our set of experiments to isolate biologically active substances from ginseng callus, suspension, and root cultures resulted in selecting the optimal extraction parameters and analyzing the extracts for the presence of organic compounds. |
| Databáze: | OpenAIRE |
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