Discrimination of extra-virgin-olive oils from different cultivars and geographical origins by untargeted metabolomics
| Autor: | Gabriele Rocchetti, Marco Trevisan, Federica Angilletta, Silvia Ghisoni, Daniela Farinelli, Sergio Tombesi, Luigi Lucini |
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| Rok vydání: | 2019 |
| Předmět: |
Multivariate statistics
Coumaric Acids Flavonols 030309 nutrition & dietetics Extra-Virgin Olive Oil Food metabolomics Polyphenols Sterols Traceability Biology Mass Spectrometry Anthocyanins 03 medical and health sciences 0404 agricultural biotechnology Settore AGR/13 - CHIMICA AGRARIA Cluster Analysis Metabolomics Food science Cultivar Extra-Virgin Olive Oil Olive Oil Chromatography High Pressure Liquid Flavonoids chemistry.chemical_classification 0303 health sciences Geography Food metabolomics Discriminant Analysis Polyphenols Traceability 04 agricultural and veterinary sciences Linear discriminant analysis 040401 food science Cholesterol derivatives Sterols Cholesterol Untargeted metabolomics Italy chemistry Food Science Olive oil |
| Zdroj: | Food Research International. 121:746-753 |
| ISSN: | 0963-9969 |
| Popis: | In this preliminary study, ultra-high-pressure liquid chromatography (UHPLC) coupled to quadrupole-time-of-flight mass spectrometry (QTOF) metabolomics followed by multivariate statistics was applied to discriminate nine extra-virgin olive oil (EVOO) cultivars according to their phenolic and sterolic fingerprints. The same approach was then used to discriminate EVOO samples from different geographical origins, namely six blends representative of the main growing regions in Italy (i.e., Sicily, Puglia, Umbria, Liguria, Lombardy and Tuscany). This approach allowed to putatively identify >1000 compounds, considering both polyphenols and sterols. The unsupervised hierarchical cluster analysis (HCA) and the orthogonal projections to latent structures discriminant analysis (OPLS-DA) discriminated EVOO samples according to both cultivar and geographical origin. In particular, flavonoids (i.e., anthocyanins and flavonols), hydroxycinnamic acids and cholesterol derivatives were found to be the most representative classes of compounds discriminating EVOO samples according to the two parameters (cultivar or geographical origin) selected. However, the following Venn analysis allowed to point out the discriminant markers being exclusive for cultivar or origin discrimination. In this regard, only the 9.6% of phenolics and 13.6% of sterols were in common, thus indicating that several of these discriminant compounds were exclusive of a single condition. Indeed, considering that most of the commercial EVOOs are blended, the contribution of both the cultivars used and the geographical origin must be taken into account. |
| Databáze: | OpenAIRE |
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