Antibody−Antigen Exchange Equilibria in a Field of an External Force: Design of Reagentless Biosensors
| Autor: | Cary J. Miller, Marcin Majda, Christopher F. Monson, Eliot Bennion, Laura N. Driscoll |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Immunoassay
chemistry.chemical_classification Magnetic tweezers Analyte medicine.diagnostic_test Field (physics) Myocardium Biomolecule Troponin I Nanotechnology Biosensing Techniques Equipment Design Sensitivity and Specificity Microspheres Analytical Chemistry Microsphere Magnetics chemistry medicine Antibody antigen Humans Biosensor |
| Zdroj: | Analytical Chemistry. 81:7510-7514 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac9010759 |
| Popis: | This correspondence presents a new strategy for detecting biological molecules that relies on competitive exchange interactions of an analyte with two-component molecular tethers attaching superparamagnetic microspheres (4 microm in diameter) to a sensor surface. The individual tethers consist of an antibody-antigen complex and are designed to selectively detect antigenic proteins in a sensitive reagentless fashion. In order to impart a driving force to the otherwise free energy neutral antibody-antigen exchange equilibrium, a small mechanical force of approximately 10 pN was applied to stretch the antibody-antigen tethers using a massively parallel magnetic tweezers device. The experimental work was carried out with human cardiac troponin I. This serum heart attack marker was used as an example of analytes of credible relevance to biomedical diagnostics. The initial results illustrate the functioning of a cardiotroponin sensor and offer a preliminary estimate of its sensitivity of 16 pM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|